Objective To retrieve,evaluate and summarize the evidence related to blood collection via central venous access devices for clinical references. Methods Systematic search was conducted in related websites and databases. The search period was from the establishment of the databases to December 7,2021,and the clinical decisions,systematic reviews,guidelines,evidence summaries,and expert consensuses were included. The quality of the literature was independently evaluated by evidence-based trained investigators and combined with professional judgment to extract information from the literature that met the quality standards. Results A total of 7 articles were included,including 6 guidelines and 1 systematic review. The best evidence included a total of 27 pieces of evidence in 4 areas,namely training of health care personnel,blood collection methods,collection of special samples,and quality control strategies for blood collection. Conclusion In the process of evidence transformation,medical staff should comprehensively consider the clinical contexts and individual factors of patients,and choose the best evidence,optimize the clinical behavior of blood specimen collection through central venous access devices,so as to improve the standardization and safety of blood specimen collection.